Although first associated with oral candidiasis in HIV-infected patients, Candida dubliniensis, one of the recently identified patogens has also been recovered from various specimens from different anatomical sites in HIV-negative individuals. However since C. dubliniensis also produces germ tubes and chlamydospores and shares the phenotypic characteristics with Candida albicans, it is really difficult to differantiate them from each other. Although molecular tests are reliable but they are not yet used routinely by many clinical microbiology laboratories. In this study we evaluated 195 isolates which were previously identified as C. albicans, with three different methods according to previously published studies to find out C. dubliniensis. By using the first method; after incubation at 37ºC and 45ºC all of the isolates which were inoculated into two seperate Sabouraud Dextrose Agar (SDA) plates were evaluated for growth. The isolates were inoculated into methyl blue included SDA for the second method and CHROMagar Candida (CAC) for the third method. After incubating for 48 hours; methyl blue included SDA were evaluated under UV lamp for detecting fluorescence of the colonies, and CAC were evaluated according to the color of the colonies. According to the method 1, 2, and 3, 85.6%, 15.9%, and 14.3% of the isolates were identified as C.dubliniensis respectively. The aggreement rates of the fluorescence detection in methylblue SDA and the color development in CAC were 94.4%, the ability to growth at 45ºC and the detection of the fluorescence were 29.2%, and the ability to growth at 45ºC and the color development in CAC were 27.7%. Differentiation of C.dubliniensis from C.albicans isolates according to the detection of fluorescence of the colonies which were inoculated into methyl blue SDA is low cost, easy to prepare and evalute and it could be recommended to use routinely by clinical microbiology laboratory but results must be confirmed by molecular techniques