Gram negative bacilli, especially Escherichia coli and Enterobactericea group, are the most frequently isolated microorganisms in urinary tract infections. It is aimed to determine the resistance pattern of E.coli isolated from urine samples . From patients with the pre-diagnosis of urinary tract infection in Manisa Government Hospital 257 E.coli isolates were obtained from urine samples between January 2000 and August 2003. Susceptibility of the 257 E.coli strains to various antimicrobial agents were tested by using the Kirby-Bauer disc diffusion method according to NCCLS standards. Of 257 strains 80 % were resistant to ampicillin , 43.2% to amoxycillin clavulanic acid(AMC), 80% to cephalotin, 30.7%, to cefuroxime, 19.5% to ceftriaxone, 1.2% to cefaperazon-sulbactam(SCF), 37.3% to trimethoprim-sulphamethoxazole(SXT),40.8% to chloramfenikol , 35.4 % to ofloxacin, 18.3 % to ciprofloxacin, 31.9 % to gentamycin, 7.4 % to amikacin. All strains were sensitive to imipenem . It's detected that strains isolated from ürine samples of hospitalized patients were more resistant than strains isolated from community acquired infeçtions (p<0.001), and that urinary system infections in patients of 50 year old or more was more frequently than in the other age groups(p<0.05).