Cryptococcus neoformans naturally colonize in avian guano and these might be a source for human infections. In this study, the presence of C.neoformans was screened in farmed ostrich (Struthio camelus) guano. Guano samples of farmed ostrich were collected from Denizli, Muğla, Isparta, Mersin and Antalya regions. No Cryptococcus neoformans isolation has been observed from 71 guano samples on Staib agar. It is considered that ostrich guano have not important to ecologically colonization thought out of large sampling area.