In this study, isolation of C. trachomatis using Mc Coy cell culture method in different clinical specimens (234 cervical, 282 urethral and 44 rectal) which were collected from 560 study participants was aimed.

Of 560 study participants; 138 unregistered sex workers, 96 registered sex workers, 44 male homosexuals and 282 male patients who had sexual contact with sex-workers were composed.

C. trachomatis was isolated in 45 (19.2%) out of 234 cervical specimens, in 43 (15.2%) out of 282 urethral samples and in 8 (18.2%) out of 44 rectal specimens.

C. trachomatis frequency was detected 16.7% in unregistered sex-workers, 12.5% in registered sex-workers, 18.2% in male homosexuals and 15.2% in men who had sexual contact with sex-workers.