Staphylococci cause difficult-to-treat infections with a substantial morbidity and mortality. The recent increase in the prevalence of methicillin resistant staphylococci (MRSA) have led to serious problems in the treatment of nosocomial infections. In this study, susceptibilities of 436 staphycoccal isolates from 28 different centers in Turkey against methicillin, fusidic acid, ofloxacin, erythromycin, ampicillin /sulbactam (SAM) and trimethoprim /sulfphamethoxazole (TMP/SMX) were tested by using disc diffusion techique. Three hundred twenty-two (74%) isolates were identified as S.aureus, and 114(26%) as coagulase negative staphylococci (CNS). Of all the isolates, 262 (60%) were resistant to methicillin. Ahigher percentage of S. aureus isolates were resistant to ofloxacin(63%) and SAM (74%) compared to CNS, whereas CNS showed a higher resistance rate to TMP/SMX (52%). 3 % of the MRSA and 13 % of the meticillin resistant KNS (MRKNS) strains were found to be resistant to fusidic asid, whereas no resistant strain to fusidic acid was found among meticillin sensitive S. aureus (MSSA) and meticillin sensitive CNS (MSCNS) strains.