Tetanus occurs worldwide and is endemic in developing countries, but it's incidence varies considerably. The most common form, neonatal tetanus, kills many infants each year because the mother was not immunized. In addition, many of the unimmunized people worldwide die each year from tetanus disease. In this study, we studied the antitoxin levels for tetanus by micro EIA in blood samples of 143 subjects aged between 17-72 and 50 controls in Manisa region. We found antitoxin levels in 107 subjects (74.8 %) were protective levels (positive); in 12 (8.4 %) were weak positive and in 24 (16.8 %) were under the protective levels (negative). All of the control subjects were found as highly positive. Tetanus protective immunity level was higher in between 17-35 years age group than 36 and older ones, and the result was statistically significant (p=0.001). In conclusion, for prevention of tetanus disease an effective immunization programme should be provided during all period of life. We consider that the vaccination should be done every 10 years for people who have had enough antitoxin levels after active immunization.