Mucosal surfaces are the first line of defence against microbes. Bacteriel infection trigger mucosal cytokine secretion. The mucosal cytokines derived, in part, from epithelial cells. Epithelial cells also produce antimicrobial peptides. In this study, the cytokine and chemokine responses and bactericidal activity of the urinary system epithelial cells stimulated with different concentration of E. coli were assesed in vitro. The cytokine and chemokine levels were determined by ELISA and bactericidal activity was detected by colony counting assay. It was observed that in epithelial cells stimulated by E.coli the levels of MCP-1, IFN- γ and IL-6 cytokines increased compared to the samples that were not included epithelial cell (p<0.05). Bactericidal effect of the urinary epithelial cells stimulated by E.coli was also determined. In the light of these findings, it is considered that urinary system epithelial cells may play an active role in the regulation of immune response of a host.