2008, Cilt 38, Sayı 1, Sayfa(lar) 005-011 |
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Study of beta lactamases in nosocomial Pseudomonas aeruginosa strains with multiple resistance |
Nigar Çelik |
İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi Çocuk Sağlığı ve Hastalıkları Anabilim Dalı Mikrobiyoloji Laboratuvarı, Cerrahpaşa, İstanbul. |
Keywords: P.aeruginosa, multiple resistance, beta lactamases. |
Beta lactamase enzymes in 50 multiply resistant Pseudomonas aeruginosa isolates from 24 pediatric and 26 adult patients
hospitalized in Istanbul University, Cerrahpaşa Faculty of Medicine were investigated. Susceptibilities of the strains were tested
with the agar dilution method. Nine (18%) strains were positive for the extended spectrum beta lactamases (ESBL) with the
classical double disk synergy test. pI ranges of the beta lactamases were between 4.5 and 8.2 by isoelectric focussing. PER-1
enzyme was positive in 22 (44%), OXA-10 derivatives in 23 (46%) and OXA-2 derivatives in 14 (28%) strains by PCR.
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