2008, Cilt 38, Sayı 1, Sayfa(lar) 037-041 |
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Evaluation of diagnostic autoantibody tests used in clinical laboratories |
Zeki Yumuk, Şeyda Çalışkan |
Kocaeli Üniversitesi Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı |
Keywords: Autoantibody, anti nuclear antibody, dsDNA |
Anti nuclear antibody (ANA) tests are valuable in the diagnosis of autoimmune disease such as systemic lupus erythematosus. Indirect
immunofluorescence (IIF) assay have been used more than 40 years for ANA tests. Because the use of IIF is time-consuming
and requires highly trained personnel considerable effort has been put into developing simpler assays for routine use. However,
newer tests can never be rated better than the old. Thus, laboratories performing autoantibody tests should continuously
evaluate the newer alternative tests. In the present study, the classical autoantibody tests and the newer alternatives in our routine
laboratory were evaluated. In conclusion, dsDNA test should be confirmed with a second test. For dsDNA, primarily IIF or RIA
should be preferred and then confirmed with ELISA method. Titration should be performed to homogenous pattern positive results.
ENA immunoblotting method might be used as a screening method and confirmation should be reached with an ELISA method.
In a clinical laboratory, before performing autoantibody tests, a comparable study concerning commercial and methodological
features should be done.
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