Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim  
2008, Cilt 38, Sayı 2, Sayfa(lar) 052-055
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The comparison of two methods in order to show the quorum sensing responses
Vahide Bayrakal, Hüseyin Baskın, İ. Hakkı Bahar
Dokuz Eylül Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı; İnciraltı, İzmir
Keywords: Pseudomonas aeruginosa, Quorum sensing, acylated homoserine lactone

Two different “quorum sensing” (QS) systems were defined in Pseudomonas aeruginosa: rhl and las. The signal molecules of the las (3- oxo-C12-HSL) and rhl (C4- HSL) systems are visualized by two different reporter strains ( Choromobacterium violaceum for rhl , and Agrobacterium tumefaciens for las).

In order to show the existence of QS systems, micro AHL, thin layer choromotograpy, zymography, and diffusion methods are used.

In this study, QS responses of two clinical isolates and standart P. aeruginosa strain (ATCC 27853) were compared by diffusion and micro AHL methods. As a result, micro AHL method was much easier to apply and to visualize, and was easier to repeat when compared to diffusion method. This may be the reason for widely applying this method while showing QS.


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Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim