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2008, Cilt 38, Sayı 2, Sayfa(lar) 083-086
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Comparison of different systems for identification of Candida strains
Nimet Yiğit1, Ayşe Esin Aktaş2, Hakan Uslu2
1 Atatürk Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu, Tıbbi Laboratuvar Bölümü, Erzurum
2 Atatürk Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji ABD, Erzurum
Keywords: Candida Species, Identification, Microbial Identification System

Chromatographically analyzes cellular fatty acids and compares the results with the fatty acid profiles in its database. Candida strains were identified as and included Candida albicans (n=74), Candida tropicalis (n=28), Candida glabrata (n=7), Candida parapsilosis (n=3), Candida krusei (n=4) and Candida guilliermondii (n=2). In preparation for fatty acid analysis, yeast isolates were subcultured onto sabouraud dextrose agar and were incubated at 28 °C for 24h. The colonies of yeast were saponified, methylated, extracted, and chromatographically analyzed according manufacturer's instructions. Of the 118 isolates tested, 79 (66,9%) were correctly identified to the species level, with 27 ( 22,8%) being incorrectly identified and 12 (10,1%) giving no identification.

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