Th e aim of this study was to determine the incidence of macrolide and lincosamide resistance phenotypes among staphylococci isolates from various clinical samples. Between January 2006 and March 2007, 436 staphylococcal isolates obtained from clinical in- and outpatient specimens at the Department of Microbiology and Clinical Microbiology of Cerrahpaşa Faculty of Medicine were examined phenotypically to detect the rates of macrolide and lincosamide resistance phenotypes. Among Staphylococcus aureus strains, the incidence of constitutive resistance predominated (23%), specially among MRSA (47.6%). Inducible resistance mechanism (11.9%) was followed by effl ux (6%) and L phenotypes (3%). Th e distribution of macrolide resistance phenotypes among CoNS isolates revealed a higher incidence of the constitutive phenotype (26.3%), followed by “effl ux” mechanisms (17.4%) and inducible clindamycin resistance (11.4%) and L phenotype (5.4%). Both S.aureus and CoNS isolates had the constitutive phenotype predominated over the inducible phenotype (23% and 26.3% versus 11.9% and 11.4%, respectively). Effl ux-mediated resistance was higher in CoNS (17.4%) than S.aureus (6%). In conclusion, MLSB resistance has been detected in a high rate among staphylococci in our hospital. Although, constitutive resistance was the most frequently encountered pattern, inducible resistance was also detected in a considerable rate in our hospital. Th is study, shows that the double-disk test must be routinely performed in our laboratory practice and clindamycin should not be recommended in patients with infections caused by inducible resistant staphylococcal isolates.