Chlamydia trachomatis is the most common reason for tubal infertility. Th e aim of this study of was to search for the presence of C. trachomatis DNA in the endoservical samples of infertile women and to evaluate the results by investigating the tubal pathologies of the patients. A total of 215 endoservical swap samples, routinely taken from infertil patients who were admitted to Gynecology and Obstetrics Clinic of the Zonguldak Karaelmas University Application and Research Hospital were included in the study. C. trachomatis DNA were investigated by Polymerase Chain Reaction (PCR) in these samples. Tubal pathologies of the patients were evaluated by examining the results of histerosalpingografi, ultrasonography and laparoscopy. Endoservical C. trachomatis DNA were found to be positive in 12 of the patients (5.6%), while negative in 203 of the patients (94.4%). Tubal obstruction were detected in three patients (25%) while no tubal obstruction were detected in nine patients (75%) out of the 12 patients who were identified as positive for C. trachomatis DNA. According to the results of the tubal investigations; tubal occlusions were determined in 13 patients (double-sided tubal obstruction in eight patients, single-sided obstruction in five patients). Th ree patients were found to be positive for C. trachomatis DNA out of the patients with doublesided tubal obstruction. In five patients who had one-sided tubal obstruction, C. trachomatis DNA was not detected. On the other hand, in nine of the patients who had no tubal obstuction, C. trachomatis DNA were identified as positive. Th e sensitivity of the PCR in detection of tubal occlusion was found as 23% while the specificity was 95%. In conclusion, PCR showed high specificity but low sensitivity in determining the tubal occlusions in infertile patients. Detecting C. trachomatis DNA in genital samples by PCR technique would help and guide the diagnosis and treatment of C. trachomatis infections. However, the cost eff ectiveness of the test must be supported by extensive studies.