2010, Cilt 40, Sayı 1, Sayfa(lar) 022-026 |
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Determination of the in Vitro Effect of Deuterium Depleted Water on Polymorphonuclear Leukocyte Functions in Healthy Young Volunteers |
Ümran Soyoğul Gürer1, Koray Derici1, Burçak Gürbüz1, Pervin Rayaman1, Adile Çevikbaş1, Turay Yardımcı2 |
1Marmara Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Haydarpaşa/İstanbul 2Marmara.Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Haydarpaşa/İstanbul |
Keywords: Deuterium depleted water, polymorphonuclear leukocytes functions, phagocytosis |
Objective: Natural water contains 150 ppm (parts per million) of deuterium (an isotope of hydrogen), which is
within the acceptable limits for most healthy people. Lowered deuterium content can affect the diseased cells in
the body while having no effect on healthy blood cells. Today it is known that frequently used antineoplastic
agents, analgesics and antibiotics affect the immune system cells and can modify polymorphonuclear leukocyte
functions such as phagocytosis, lymphocyte proliferation and production of various cytokines. The aim of this
study was to determine the effect of deuterium depleted water (29 ppm) on phagocytosis and intracellular killing
activity of polymorphonuclear leukocytes in healthy young volunteers.
Materials and Methods: 10 healthy young volunteers were included in the study. Ficoll-hypaque gradient
centrifugation was used to isolate polymorphonuclear leukocytes from venous blood. Phagocytosis and intracellular
killing activity of polymorphonuclear leukocytes were assayed using a modification of Alexander's method.
Results and conclusion: When the effect of deuterium depleted water on polymorphonuclear leukocytes were
evaluated, it was found that depletion of deuterium didn't significantly affect the polymorphonuclear leukocyte
phagocytic and intracellular killing activities.
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