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2010, Cilt 40, Sayı 2, Sayfa(lar) 087-096
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The Identification of Anaerobic Bacteria Isolated from Various Clinical Materials and Determination of Their Antibiotic Susceptibilities with E-Test Method
Recep Keşli1, Selahattin Çelebi2
1Konya Eğitim ve Araştırma Hastanesi, Mikrobiyoloji Laboratuarı, Konya
2Atatürk Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji AD, Erzurum
Keywords: Anaerobic bacteria, beta lactamase, antibiotic susceptibility

Objective: This study was aimed to identify the anaerobic bacteria isolated from various clinical materials of patients with suspected anaerobic infection and also to determine their beta-lactamase production and antibiotic susceptibilities.

Materials and Methods: The specimens transported to the laboratory under anaerobic conditions were inoculated onto Schaedler agar, Schaedler broth and chopped-meat broth and incubated in anaerobic atmosphere. Bacteria grown in these media were identified by gram staining, conventional microbiological methods and API 20 A, API 20E and API 20NE systems. MIC values of the isolated strains were determined by the E test method.

Results: Of the forty anaerobic bacteria isolated from 100 clinical specimens; 19 were gram positive non-spore forming bacteria, 11 were in the Bacteroides fragilis group, five were gram positive spore forming rods, two were black pigmented gram negative rods, two were Fusobacterium species and one was Veillonella parvula. Betalactamase production was detected in 10 strains, of which six were gram-negative and four were gram-positive rods. Of the 16 gram negative anaerobic bacteria; 11 were resistant to penicilin G, eight to clindamycin, four to cefoxitin, three to piperacillin/tazobactam and one to metronidazol. No resistance was detected against imipenem among gram negative rods. Of the 24 gram positive anaerobic bacteria resistance to penicillin G, clindamycin, metronidazol, cefoxitin, piperacillin/tazobactam, and imipenem were 16, 13, nine, six, six, and one respectively. In all the tested strains the lowest percentage of resistance was against imipenem (2.5 %).

Conclusion: These results emphasized that anaerobic bacteria should be considered as causative agents in the laboratory diagnosis of infectious diseases and imipenem seemed to be the most effective anti-anaerobic agent.


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