Objective: The importance of enterococci has been increasing in either nosocomial or community acquired infections. Not only does the isolation, identification and antimicrobial susceptibility detection but also the follow up of antibiotic susceptibility profiles has a great priority for enterocoocal infections.

Materials and Methods: In this study, antibiotic resistance rates of a total of 137 enterococcus strains isolated in Afyon Kocatepe University Medical Faculty Microbiology Laboratories were evaluated. Of the isolated strains 71 were from blood, 39 were from urine and 27 were from wound samples. Identification of bacteria and antibiotic susceptibility testing were performed by standard microbiological identification procedures and by an automated system.

Results: Resistance rates were found as 81.5 % for ampicillin, 51.9 % for imipenem, 61.1 % for ciprofloxacin and 38.9 % for moxifloxacin. No resistance was shown to vancomycin, teicoplanin, linezolid and tigecycline. High level aminoglycoside resistance was detected as 46 % for gentamycin and 44.5 % for streptomycin.

Conclusion: Despite any glycopeptide resistant strains was not detected, our study demonstrated the increasing rates of aminoglycoside resistance, and indicated the necessity to be attentive in the treatment of serious enterococcal infections.