Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2011, Cilt 41, Sayı 4, Sayfa(lar) 155-161
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The Effect of Short Time Drying and Temperature Change on Microbial Biofilm Formation in a Model Pipe Rig Feed with Distributed Network Water
Duygu BAŞ1, İrfan TÜRETGEN2
1Merck Sharp Dohme İlaçları Ltd. Şti.
2İstanbul Üniversitesi Fen Fakültesi Biyoloji Bölümü, Temel ve Endüstriyel Mikrobiyoloji Anabilim Dalı
Keywords: Biofilms, network water, epifluorescence microscopy

Objective: Aquatic microorganisms can adhere to the surfaces and develop biofilm layers. Biofilm layer can protect microorganisms against short-term drying, disinfectants, pH alterations, antimicrobial agents, toxins and viruses. Water cutbacks are unavoidable in the distributed network water. The biofilm on the inner surface of water pipes starts to dry when the system is shutdown or in case of water cutbacks. Our aim was to monitor the response of the biofilm bacteria in terms of water temperature alteration and short time drying.

Materials and Methods: In this study, biofilm formation in a model polypropylene domestic pipe rig system were allowed for an eight month-period. Pipe segments were cut monthly and the effect of 6, 24, 48, 72 hours of drying on biofilms were analyzed in terms of growth of aerobic mesophilic heterotrophic bacteria (AMHB) and the numbers of live/dead microorganisms were analyzed by epifluorescence microscopy. The amount of biofilm on pipe surfaces was analyzed quantitatively.

Results: Exposure to desiccation for 6, 24, 48, 72-hours and the drop of bulk water temperature during the eight month experimental period (from August to March) significantly reduced the AMHB in biofilm layer, however, quantitative measurement of biofilm layer and live/dead bacterial counts revealed that there were no significant differences.

Conclusion: The study showed that biofilm layer can protect microorganisms from short-term drying but in some circumstances they are not culturable with classical microbiological methods. Therefore different methods should be applied to avoid counting errors.


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Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim