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2011, Cilt 41, Sayı 4, Sayfa(lar) 162-167
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Evaluation of Different Culture Media for the Isolation of Vancomycin Resistant Enterococci
Defne GÜMÜŞ1, Deniz SERTEL ŞELALE2, Yaşar NAKİPOĞLU2, Mine ANĞ KÜÇÜKER1
1Yeni Yüzyıl Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı
2İstanbul Üniversitesi İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı
Keywords: Vancomycin resistant enterococci, colonization, media

Objective: Rapid and reliable detection of vancomycin resistant enterococci (VRE) colonized patients who may act as a reservoir for VRE associated nosocomial infections and outbreaks, is of crucial importance. Periodical screening of patients under risk for VRE infections is recommended especially for critically ill patients staying at intensive care units. This study was aimed to evaluate the performance of different culture media and methods for screening VRE colonization.

Materials and Methods: In this study 29 different agar media prepared by different concentrations and ratios of esculin, vancomycin, teicoplanin, azide glucose broth (AGB), brain heart infusion agar and NaCl were tested for direct plating method; four combinations found to be highly selective were used for direct inoculation of clinical samples and the results were compared with routinely used enrichment culture method. In addition, two enrichment culture methods (standard and modified) were compared for their ability to detect VRE from rectal swabs. Rectal swabs were inoculated onto AGB and vancomycin (6μg/ml) added azide glucose broth (AGBV). Subcultures were performed from AGBV to tryptic soy agar (TSA) and from AGB to vancomycin added TSA (VTSA) and the results were compared accordingly.

Results: One hundred and nine rectal swabs taken from hospitalized patients were studied. Among the 29 media investigated the best four were those which did not contain NaCl and teicoplanin and had 8μg/ml vancomycin and AGB. Broth enrichment method was superior to direct plating for the isolation of VRE. VRE detection rate was higher when AGBV was used (AGB 17.4%, AGBV 26.6%), however, the difference was not statistically significant.

Conclusion: Broth enrichment method increases the isolation of VRE


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