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2015, Cilt 45, Sayı 1, Sayfa(lar) 012-021
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Investigation of BK and JC Virus DNA Positivities by Real-Time Polymerase Chain Reaction in Immunosuppressive Patients
Meryem ÇOLAK1, Aylin ALTAY1, Yasemin ERTEN2, Zübeyde Nur ÖZKURT3, Seçil ÖZKAN4, Ahmet PINAR5, Gülendam BOZDAYI1
1Gazi Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara
2Gazi Üniversitesi Tıp Fakültesi, Nefroloji Bilim Dalı, Ankara
3Gazi Üniversitesi Tıp Fakültesi, Hematoloji Bilim Dalı, Ankara
4Gazi Üniversitesi Tıp Fakültesi, Halk Sağlığı Anabilim Dalı, Ankara5Hacettepe Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara
Keywords: BK-JC virus, immunosuppressive patient, real-time polimerase chain reaction

Aim: This study was aimed to investigate the BK virus (BKV) and JC virus (JCV) DNA by real-time polymerase chain reaction (Real-Time PCR) in immunosuppressive patients with high risk.

Materials and Methods: A total of 296 samples obtained from 129 patients hospitalized in Gazi University Hospital, Ankara, Turkey between March 2014-May 2015 were included in the study. Viral DNA was extracted by the “MagNA-Pure Compact NucleicAcid Isolation Kit” (Roche, Germany). By using amplification mix LightMix® BK/JC Polyomavirus Detection Kit (TIB-Molbiol, Germany) that included primers targeting JCV and BKV, nucleic acids were amplified with LightCycler® 2.0 (Roche, Germany).

Result: BKV-JCV positivity rate was found as 35.4% (105/296). In urine samples the rate of positivity was 56.5% (26/46) for BKV and 10.8% (5/46) for JCV. In blood samples the rate of positivity were 29.2% (73/250) for BKV, 3.2% (8/250) for JCV. The distribution of the units with BKV-JCV positive patients, respectively, were as follows: 59.6%-13.4% from the bone marrow transplantation unit, 32.4%-1.2% from paediatric nephrology, 16.8%-2.9% from nephrology. BKV positivity rate was 78.5% (11/14) in adult haematology and 57.1% (8/14) in paediatric haematology. One patient from paediatric haematology revealed both BKV and JCV positivity. There were no JCV positivity in patients followed at the adult haematology unit. In 25.2% (25/99) of the samples, BKVDNA was detected ≥104 copies/ml and 80% (20/25) of those samples were urine.

Conclusion: Monitorization of BKV by Real-Time PCR in kidney transplant patients is of crucial importance since high quantities of BKV is associated with graft dysfunction and organ rejection following transplantation. Follow-up of BKV nephropathy through BKV quantitation by real-time PCR, may help to decrease the detrimental effects on transplant outcome.


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