2015, Cilt 45, Sayı 3, Sayfa(lar) 128-135 |
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Investigation of West Nile Virus in Cerebrospinal Fluid and Blood Samples of Patients with Initial Diagnosis of Central Nervous System Infection and Comparison of Results |
Zülfü BAYAR, Mustafa YILMAZ, Zülal AŞCI TORAMAN, Yasemin BULUT |
Fırat Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, Elazığ |
Keywords: WNV, CSF, ELISA, RT-PCR |
Objective: West Nile Virus (WNV) was firstly isolated in
1937 in West Nile area of Uganda. This virus is an enveloped,
45-50 nm sized RNA virus with icosahedral symmetry that is
classified in the Japanese encephalitis virus serocomplex of
genus flavivirus of the family flaviviridae. The aim of our
study is to show WNV specific antibodies or viral nucleic
acid in blood and/or cerebrospinal fluid (CSF) o fthe
patients with initial diagnosis of CNS infections.
Materials and Methods: Routine blood and CSF samples
without any identifiable pathogenic microorganism of the
patients who were treated with the initial diagnosis of central
nervous system infection which were sent to the hospital's
central laboratory, were collected. Ninety-four samples of 47
patients (47 CSF and 47 blood) were examined with no
distinction of age, gender, and service. WNV were examined
by ELISA and RT-PCR methods in these samples.
Results: Average age of the patients included in this study was
19 years. CSF samples of the patients showed pleocytosis
(average 155 cells), moderately high protein levels (mean 89
mg/dl) and mildly low glucose levels (mean 53 mg/dl). In this
study, only in one patient's blood sample suspect WNV IgM
positivity was detected by ELISA. In all other samples WNV
IgM and IgG antibodies were not detected. The CSF and
blood samples of the suspected WNV IgM positive samples
were found to be negative by RT-PCR method. Similarly, all
samples were tested by RT-PCR method and no RNA related
to WNV was detected.
Conclusion: In our study, suspect positive result was
considered to be related with cross reactivity that can be
detected in ELISA method. BNV seronegativity mostly
resulted from the low mean age level (mean: 19) of the
patients included in the study. Also according to the data
we obtained in our study with PCR and ELISA, WNV borne
CNS infections were not encountered in our region.
Depending on the ecological conditions more comprehensive
studies will be needed. We hope our work is of benefit for
the WNV database in our country.
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