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2015, Cilt 45, Sayı 4, Sayfa(lar) 181-187 |
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Investigation of Biofilm Formation of ESBL Positive Uropathogenic Escherichia coli Isolates on (Microplate) Surfaces Modified with Plasma Polymerization Technique and Nanomaterials: An Experimental Model |
Elvan HORTAÇ1, Gizem KALELİ2, Dilek ÇÖKELİLER3, Şükran YAVUZDEMİR4, Mehmet MUTLU2, Müge DEMİRBİLEK EKİCİ1, Jülide Sedef GÖÇMEN1 |
1Başkent Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara 2TOBB ETÜ Mühendislik Fakültesi Biyomedikal Mühendislik Bölümü, Ankara 3Başkent Üniversitesi Mühendislik Fakültesi Biyomedikal Mühendislik Bölümü, Ankara 4Atatürk Hastanesi Mikrobiyoloji Laboratuvarı, Ankara |
Keywords: Biofilm, nanomaterial, uropathogenic E. coli |
Objective: Extended spectrum beta lactamase (ESBL)
positive urinary pathogen Escherichia coli is one of the
major agents that lead to catheter-associated urinary tract
infection. Prevention of colonization via coating the
catheter with biocompatible materials produced by
biotechnology is an important step in the prevention of
infection. In the presented study, it was aimed to investigate
the biofilm formation of ESBL positive urinary pathogen E.
coli isolates on microplates which were coated with
different bio-compatible nano-materials.
Materials and Methods: Biofilm formation of a total of 45 ESBL positive E. coli isolates identified at urine cultures of patients who attended to the medical and surgical outpatient clinics of Ankara TOBB ETU Hospital, on microplates coated with two different monomers [2- hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDM)] using plasma polymerization technique was examined. Results: Out of 45 ESBL positive E. coli strains used in the study, 8 were observed to form a slight level of biofilm on uncoated plates whereas on EGDM coated microplates 11, and on HEMA coated microplate only 1 strain formed biofilm. Conclusion: In vitro results showed that using urinary catheters coated with monomer material can reduce catheter associated urinary system infections and morbidity and mortality rates accordingly. |
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