Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim  
2016, Cilt 46, Sayı 3, Sayfa(lar) 112-121
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Efficiency of BD MAXTM CRE Method on Detection of Carbapenemase-Producing Enterobacteriaceae spp. from Rectal Swab Samples
Ayşe Nur SARI1,2, Sema ALP ÇAVUŞ3, Zeynep GÜLAY1
1Dokuz Eylül Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı
2Sanko Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı
3Dokuz Eylül Üniversitesi Tıp Fakültesi, Enfeksiyon Hastalıkları ve Tıbbi Mikrobiyoloji Anabilim Dalı
Keywords: Rapid screening tests, Carbapenemase producing Enterobacteriaceae, NDM/OXA-48/KPC

Objective: BD MAXTM CRE is a real-time polymerase chain reaction (PCR) assay kit which determines KPC, OXA-48 and NDM genes where all steps like DNA extraction are automatized. Twenty- four samples can be loaded to the system in one run and results can be obtained in 2.5-3 hours. In this study, our aim was to investigate efficiency of BD MAXTM CRE assay's by comparing the system with in-house method used in our molecular epidemiology laboratory.

Material and Method: Carbapenemase- producing Enterobacteriaceae (CPE) were screened in rectal swab samples of patients hospitalized in units with increased risk for nosocomial infection. Both BD MAXTM CRE assay and culture followed by an “in-house” PCR method already in use in our molecular epidemiology laboratory, were performed. Forty-six rectal swab samples were included in the study and also two samples with predetermined carbapenemase gene types were included as positive controls in each run.

Results: When evaluation based on any carbapenemase gene positivity in Enterobacteriaceae members was performed, no carbepenemase genes were detected in twenty-six samples by both methods. In although different enzyme types were identified in fifteen samples, at least one carbapenemase gene was found by two methods. In three samples, carbapenemase genes were detected only by the “in house” method following the bacterial culture and for the remaining two only by BD MAXTM CRE assay. Sensitivity, specificity, positive, and negative predictive values for BD MAXTM CRE assay were 88, 90, 83, and 93%, respectively.

Conclusion: Although further studies are needed to determine the efficiency of BD MAXTM CRE system. Our results indicate that BD MAXTM CRE Assay is suitable for use in that it enables rapid detection of carbapenemase–producing Enterobacterteriaceae strains, and carriers.


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