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2005, Cilt 35, Sayı 2, Sayfa(lar) 123-125
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Comparision of PCR and EIA Results in Diagnosis of HCV Infection
Tuncer Özekinci, Selahattin Atmaca, Nezahat Akpolat, Hakan Temiz, Eralp Arıkan
Dicle Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Diyarbakır
Keywords: HVC RNA, Polymerase Chain Reaction (PCR), EIA

The aim of the present study was to investigate the positivity of HCV RNA by polimerase chain reaction (PCR) in patients with positive and negative anti-HCV. The sera of patients sent to the Routine Laboratory, Dicle University Hospital were studied. Serum samples obtained from 95 patients with suspected HCV infection were analyzed. Anti-HCV was found to be positive in 178 ( 69.8%) and negative in 77 (30.2%) of the patients. HCV RNA was found to be positive 123 (48.3%) of the patients. Anti-HCV was found to be positive 108 (87.8%) and negative in 15 (12.2%) of patients with positive HCV RNA. It was found that detection of HCV RNA in anti-HCV negative patients were important as in positive patients. Because of that in routine laboratories PCR seemed to be a valuable assay for HCV RNA determination for diagnosis and also following up therapies.

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