2016, Cilt 46, Sayı 4, Sayfa(lar) 152-158 |
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Viral Agents Identified in Cerebrospinal Fluid Samples |
Rabia CAN SARINOĞLU, İmran SAĞLIK, Derya MUTLU, Betil ÖZHAK BAYSAN, Dilara ÖĞÜNÇ, Dilek ÇOLAK |
Akdeniz Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Antalya |
Keywords: CSF, PCR, Viral CNS infection |
Objective: Viral infections of central nervous system (CNS)
are rapidly progressive, acute infections with high mortality
and morbidity rates, and affect people of every age. Polymerase
chain reaction (PCR), which is a nucleic acid amplification
test (NAAT) is frequently used for diagnosing viral infections
of CNS. The aim of this study is to retrospectively analyze the
results of cerebrospinal fluid (CSF) samples, which were sent
to Akdeniz University Medical Faculty Hospital, Central
Laboratory, Department of Microbiology to be investigated
for viral agents by PCR method.
Material and Methods: The results of a total of 2849 CSF
samples from 704 patients, sent to Akdeniz University
Hospital Central Laboratory, Department of Microbiology
between 2010-2014 with a preliminary diagnosis of viral
CNS infection, were retrospectively analyzed. The CSF
samples were tested by the real-time PCR method for
adenovirus (AdV), cytomegalovirus (CMV), Epstein Barr
Virus (EBV), enterovirus (EV) and herpes simplex viruses
type 1 and type 2 (HSV- 1, HSV- 2). Also, protein and
glucose levels in CSF samples were recorded.
Results: Thirty eight CSF samples from 35 patients were
found to be positive for viral infectious agents. EBV DNA,
EV RNA, HSV-I DNA, AdV DNA, CMV DNA and HSV-2
DNA were detected in 3.6% (11/308), 1.8% (8/447), 1.7%
(12/721), 1% (4/405), 0.4% (1/271) and 0.2% (2/697) of
the samples respectively. HSV-I DNA and EBV DNA were
detected in one patient simultaneously. The CSF glucose
levels of the patients, who were found to be positive for
viral infectious agents were within normal limits in 19
(54.2%), low in six (17.1%) and high in ten (28.6%)
patients. Also normal, and higher CSF protein levels were
found in 17 (48.6%) and 18 (51.4%) patients, respectively.
Conclusion: Using PCR based molecular methods for the
investigation of all possible viral agents in CSF samples
will conceivably provide opportunity for early diagnosis
and therapy of viral CNS infections.
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