Cryptococcosis is caused by Cryptococcus neoformans, a capsule-containing yeast. It enters the body predominantly through inhalation of airborne fungal elements from an environmental source. It frequently causes infections specifically in immunosuppressed patients. The yeast mainly invades central nervous system (CNS) and the lungs, although widespread (disseminated) disease may develop. Meningitis that develops as a result of the invasion of the CNS, may not present with the classical symptoms. Analysis of cerebrospinal fluid (CSF) shows lymphocytic pleocytosis, increased protein , and reduced glucose levels in meningitis cases. The samples are stained with Indian ink or nigrosin stain. In Indian ink preparations, white budding organisms are observed as colorless buddings against the dark background. Culture is needed for definitive diagnosis. CSF samples are cultivated on blood agar, Sabouraud dextrose agar (SDA) without cycloheximide and liquid SDA. Culture plates are incubated at 37°C for 24 hours. In our study we investiagted six cryptococcosis cases isolated in microbiology laboratory over 12 years. Information of patients were obtained by examining patient files. It was determined that two patients initially received treatment for TB meningitis. Two patients were HIV positive and four of the six patients were lost because of the infection.