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2017, Cilt 47, Sayı 4, Sayfa(lar) 205-212
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Investigation of Bacterial Meningitis Agents from Patients with Suspicion of Non-Neonatal Meningitis by Conventional and Molecular Methods
Mehmet YARYÜZLÜ1, Gönül ASLAN2, Mahmut ÜLGER3, Seda TEZCAN ÜLGER1, Gürol EMEKDAŞ4, Necdet KUYUCU5, Ali KAYA6
1Mersin Halk Sağlığı Laboratuvarı, Mersin
2Mersin Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Mersin
3Mersin Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Mersin
4Biruni Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul
5Mersin Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Mersin
6Mersin Üniversitesi Tıp Fakültesi, Klinik Bakteriyoloji ve Enfeksiyon Hastalıkları Anabilim Dalı, Mersin
Keywords: Acute bacterial meningitis, culture, polymerase chain reaction, latex agglutination test

Objective: In the most of purulent meningitis cases Haemophilus influenzae, Streptococcus pneumoniae and Neisseria meningitidis are responsible. In this study we aimed to identify our these nonneonatal bacterial meningitis agents isolated in our hospital.

Material and Methods: Cerebrospinal fluid (CSF) specimens of 137 suspected cases of non-neonatal meningitis from various clinics of Mersin University Health Research and Application Center were included in this study. CSF specimens were subjected to Gram staining, cell count and conventional cultures. Serotyping of the strains isolated from culture was done. For the rapid diagnosis of these bacterial agents polymerase chain reaction (PCR) and latex agglutination test (LAT) were performed with the CSF specimens.

Results: The study population consisted of 87 (63.5%) male, and 50 (36.5%) female patients. Five gram positive diplococci were detected by Gram strain from CSF specimens. Culture was positive in four (2.9%) specimens (S. pneumoniae in 3, and H. influenzae in 1), PCR was positive in 18 (13.1%) and LAT was positive in 17 (12.4%) specimens. With these three methods 19 (13.8%) patients were diagnosed as bacterial meningitis. Serotypes of S. pneumoniae isolates were identified as 6A/B, 23F and 8; and serotype of H. influenzae isolates as type b.

Conclusion: Though culture is indispensable for the bacterial isolation and determination of antibiotic sensitivity; however, usage of molecular methods and LAT with culture would be very useful for rapid diagnosis of bacterial meningitis especially in patients with prior antibiotic usage.


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