2018, Cilt 48, Sayı 2, Sayfa(lar) 100-111 |
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|
The Evaluation of Biological Remediation Potential of Bacteria Isolated from Marmara and Black Sea for Degradation of Crude Oil and Oil Derivatives |
Yosun MATER, Selçuk TAŞDAN |
Gebze Teknik Üniversitesi, Temel Bilimler Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Gebze, Kocaeli |
Keywords: Bioremediation, marine microbiology, crude oil, oil contamination |
Objective: Decontamination of petroleum and its derivatives from
natural sources is a very critical task due to highly polluting
properties of the petroleum. Removal or conversion to different less
toxic or non-toxic forms of oil hydrocarbons via bacteria, known as
bioremediation has become an important research area. In this
study, the aim was to determine the bacteria which have the abilty
of degradation of oil and oil derivatives by scanning strains of 698
marine bacteria isolated from Marmara and Black Sea.
Material and Method: Within this context, firstly, 7 selected
isolates known to degrade oil were incubated for 30 days at
different concentrations and larger scale in crude oil and thickness
of the oil layer in millimetres and morphological changes observed
were recorded on a daily basis. After 30 days of experimental
period, samples were prepared for gas chromatography-mass
spectrometry analysis. In the last phase of the study, seven
bacterial isolates were tested by the VITEK 2 Compact Automatic
Identification System for pre-identification.
Results: Based on experimental results, bacteria were able to survive
and degrade the crude oil even at the highest petroleum concentrations.
The highest reduction in the petroleum layer thickness was observed
for 333, A87 and 331 isolates. Quinoline (C9H7N) was detected as the
degradation product of petroleum, in all isolates. VITEK system
predefined 5 isolates including Serratia plymuthica, Enterococcus
faecalis, Klebsiella pneumoniae subsp. ozaenae, Staphylococcus
lentus, and Staphylococcus sciuri. As a result the candidate bacteria
that may be used for biological remediation of pollution of our sea
arising from oil have been determined.
Conclusion: The study is one of the original and pioneering works
in terms of showing that existing bacteria in Marmara and Black
Sea can be used for bioremediation purposes.
|
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|