Objective: Brucellosis is a systemic infectious disease that is commonly detected in many countries of the world and is very common in our country. The aim of this study was to evaluate the serological markers of brucellosis in 6045 patients from Istanbul and surrounding provinces, who were admitted to our center in a 4-year period and to compare the results with the study conducted between 2005-2011.

Method: Serum samples of suspected brucellosis patients who were admitted to Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty Hospital, Serology / ELISA unit, between March 2013-2017 were included in the study. Brucella Serum Agglutination, Coombs Wright and Rose-Bengal tests were performed.

Results: As a result of a 4 years evaluation, 107 (1.8%) of the 6045 suspected brucellosis cases were found seropositive and 5938 (98.2%) of them were seronegative. Of the seropositive 107 cases, 73 (68.2 %) were found to be concurrently positive with RB and SAT; 34 (31.7%) of the cases were seropositive according to CT. Although brucella seropositivity rate was higher in females (58%) than males (42%), no statistically significant difference was found. Of the cases 28% had transmission from the same source within the family and 48% had a history of living in rural areas.

Conclusion: Consequently, the seropositivity rate of brucellosis (1.8%) that we found was found to be lower than the rate obtained in the same center (3%) previously. We believe that, by the increase in visual and written communication tools people comply and are more conscious about the hygienic rules on consuming animal based nutrition.