We evaluated all bacteremic epizods over a 12 month period between January 2003 and December 2003 at Central Microbiology Laboratory of Akdeniz University Hospital. Seven hundred and sixty bacteremic epizodes were identified in 9295 hospitalized patients. A total of 807 bacteria were isolated from 760 bacteremic epizodes. Of the 807 bacteria, 54.4% were Gram positive and 44.6% were Gram negative respectively. Coagulase Negative Staphylococcus (CNS) were the most frequently isolated species (25.9%), followed by Staphylococcus aureus (S. aureus) (14.7%) and Enterococcus sp. (10.6%). Sixty three percent% of CNS and 42% S.aureus isolates were resistant to methicillin. No resistance for glycopeptides was observed among Enterococcus sp., Escherichia coli (E. coli), Pseudomonas sp. and Acinetobacter sp. were the most frequently isolated Gram negative bacteria and the most effective antimicrobial agents for those isolates were carbapenems (100%), piperacillin- tazobactam (92%) and sefoperazon-sulbactam (88%) respectively.