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2020, Cilt 50, Sayı 2, Sayfa(lar) 095-099 |
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Investigation of the Mating-Type Gene Locus of Candida albicans |
Müge Arıbal Çelik1, Aylin Döğen1, Deniz Aktaş1, Süleyha Hilmioğlu Polat2, Macit İlkit3 |
1Mersin Üniversitesi Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Mersin, Türkiye 2Ege Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İzmir, Türkiye 3Çukurova Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Adana, Türkiye |
Keywords: Candida albicans, sexual reproduction, MAT locus, virulence |
Objective: Mating in Ascomycete fungi is governed by mating type gene region called
the MAT locus. The MAT locus contains idiomorphic sequences that are dissimilar in
two opposite mating types.In fungi belonging to the Pezizomycotina subphylum, each
MAT idiomorph encodes an alpha domain and an HMG (High Mobility Group) domain
transcription factor. Although the alpha domain and the HMG domain transcription
factor genes are indispensable components, the structure of the MAT locus (length),
the gene content, and the transcriptional directions of the genes differ among fungi.
This study aimed to evaluate the characterization of mating type of Candida albicans
isolated from blood samples in our labouratory culture collection.
Method: Using the multiplex PCR technique, 486 bp and 515 bp of the DNA region of MTLa and MTL? primers in the C. albicans genome were determined, respectively. Results: 156 C. albicans isolates showed 155 MTLa/? (99.4%) and 1 MTL?/? (0.6%). Conclusion: Determination of MTL is essential both for making molecular genetic studies more efficient and for understanding the nature of this pathogenic fungus. |
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