2020, Cilt 50, Sayı 3, Sayfa(lar) 164-171 |
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|
In Vitro Evaluation of the Increase in MIC Value of Colistin in the Carbapenem Resistant Klebsiella pneumoniae Strains Over the Years |
İsmail Selçuk Aygar |
Sağlık Bilimleri Üniversitesi, Gülhane Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Ankara |
Keywords: Carbapenem-resistant Klebsiella pneumoniae, colistin resistance, colistin MIC level |
Objective: Klebsiella pneumoniae can be found colonized on human skin, gastrointestinal flora
and nasopharynx. We aimed to examine the increase level of minimal inhibitory concentration
values of colistin is alternative treatment in carbapenem resistant Klebsiella pneumoniae and to
compare the results of automated system with gold standard fluid microdilution method of
colistin sensitivity.
Method: Sixty-six carbapenem resistant Klebsiella pneumoniae strains isolated from clinical
samples between January 2015-July 2017 were included in the study. Carbapenem susceptibilities
of strains studied with automated system were also tested by disk diffusion method. To measure
colistin sensitivities, liquid microdilution test was studied using colistin sulfate active substance in
accordance with EUCAST recommendations. The MIC range for colistin was studied with values
between 0.125-64 ?g/ml. Chi-square test was used to analyze the data.
Results: A total of 24 (36.4%) isolates, 6 (37.5%) in 2015, 8 (35.8%) in 2016 and 10 (41.7%) in
2017, were found to be resistant to colistin. No statistically significant difference was found in the
MIC values of colistin over the years (p>0.05). There was discrepancy between the results found
by two different methods. Very major errors were detected in two isolates and major errors were
detected in two isolates.
Conclusion: It is worrisome that carbapenem resistant Klebsiella pneumoniae isolates were
detected in about one-third of colistin resistance. Also, it?s important to determine the resistance
rates with gold standard fluid microdilution method. This reduces the treatment options alone or
in combination, suggesting that these isolates will become more difficult to treat in future and
new treatment strategies should be studied.
|
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|