Objective: It was aimed to determine the knowledge and attitudes of health program students studying at Cappadocia Vocational School about antibiotic use and bacterial resistance.

Methods: This is a descriptive and cross-sectional study. The population of the research was formed by the students studying in health programs in the 2019-2020 academic year. The students who accepted to participate in the study and completed the questionnaire fully (n=350) was included in the sample. The forms were sent to the participants electronically.

Results: Hundred and eighty-six (53.1%) participants agreed that antibiotics accelerated recovery from the common cold, and 75.7% (n=265) of the participants were aware that the antibiotics could play a role in combating bacterial resistance. The most important source of information on antibiotics was doctors/nurses in 68% (n=238) of the participants. Most (91.7%) of the participants (n=321) reported that the population of Turkey used antibiotics unnecessarily and 83.4% (n=292) of them advocated that the treatment should be complete. While there is no significant relationship between the knowledge level of the participants and their gender; a statistically significant relationship was found between education and knowledge levels of the participants.

Conclusion: As a result of this study, it was determined that the students have a moderate knowledge about antibiotic use and bacterial resistance. In order for students to have sufficient information about antibiotic use and bacterial resistance organization of awareness campaigns, provision training on the subject and carrying out further studies on the this subject may be suggested.