Objective: Antibiotic resistance is one of the most significant problems of human-animal and environmental ecosystems. It is crucial to establish integrated surveillance systems and monitor resistance for the management of antibiotic resistance. Standardization of antibiotic resistance data obtained from various disciplines is the critical point in enhancing the data quality. To realize this objective, a common antibiotic susceptibility testing training program was prepared and performed for professionals from various disciplines to standardize the resistance data to be obtained from the human, animal, and environmental sectors in our country.

Method: A total of 48 individuals participated in a five-day training program in three terms. In each period, four small groups, each consisting of four people from a group of different professions, were generated. Participants were trained on quality control, phenotypic tests, genotypic tests, and the use of resistance data in antibiotic susceptibility testing. Pre-test and post-tests were applied to the participants.

Results: Individuals with a postgraduate degree who studied antibiotic susceptibility testing in the fields of medicine, veterinary medicine, pharmacy, food, and environment participated in the training. The average number of correct answers in the pre-test and post-test increased from 4.8 to 10.5 in April, from 4 to 9 in June and from 3.4 to 8.5 in September. They studied phenotypic and genotypic tests in the supplied isolates under the supervision of the educators.

Conclusion: We presume that dissemination of the training at graduate and postgraduate levels will also enable the One-Health approach to become widespread. In addition, worldwide application of similar trainings will help standardization of resistance data, as well as one health approach.