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2023, Cilt 53, Sayı 2, Sayfa(lar) 084-092 |
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Genotyping of Mycobacterium tuberculosis Complex Isolates from Patients with Tuberculosis by MIRU-VNTR and Spoligotyping Methods |
Nurcihan Biltekin1, Mahmut Ülger1, Mediha Begüm Kayar2, Gülfer Yakıcı2, Seda Tezcan Ülger3, Gönül Aslan3, Nuran Delialioğlu3, Fatih Köksal4 |
1Mersin Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, Mersin, Türkiye 2Gaziantep İslam Bilim ve Teknoloji Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Gaziantep, Türkiye 3Mersin Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Mersin, Türkiye 4Çukurova Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Adana, Türkiye |
Keywords: Spoligotyping, MIRU VNTR, Mycobacterium tuberculosis |
Objective: Tuberculosis is a bacterial disease caused by a single infectious agent, resulting in significant mortality
and morbidity worldwide. Almost a quarter of the world’s population is infected with tuberculosis bacillus, and
about two million people die each year from this disease. In this study, it was aimed to genotyping Mycobacterium
tuberculosis compleks (MT) isolates in our province and to obtain molecular epidemiological data of these isolates.
Methods: In the study, genotyping of 103 MTC isolates in the Mersin University Faculty of Medicine Department of Medical Microbiology Mycobacteriology Laboratory was performed using spoligotyping and MIRU-VNTR methods. Results: As a result of molecular genotyping, it was observed that 103 isolates were distributed in 10 spoligotyping clusters. It was determined that the T family constituted the largest cluster with 48 (46.6%) isolates. This was followed by 16 (15.5%) isolates of the Haarlem family, 13 (12.6%) isolates of the TUR family, 10 (9.7%) isolates of the S family, four (3.9%) isolates of the Beijing and LAM families, three (2.9%) isolates of the Bovis and NEW-1 families were followed by Delhi/CAS and H37Rv families, with one (0.9%) isolate. Conclusion: As a result of the study, it was determined that the most common family was the T family with a rate of 46.6%. The Beijing family, which was reported to be associated with multi-drug resistant tuberculosis in previous studies, has now been identified in our region. It is thought that the combination of MIRU-VNTR and spoligotyping methods will be useful in the control and elimination of tuberculosis in the population in that region by monitoring the motility of M. tuberculosis in the region where molecular epidemiological studies are carried out. |
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