Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2000, Cilt 30, Sayı 1-2, Sayfa(lar) 056-059
[ Özet ] [ PDF ] [ Benzer Makaleler ] [ Editöre E-Posta ]
Isolation and Preparation of Antisera to Ovine IgG Heavy Chain
Hüseyin Yılmaz1, Nuri Turan1, Kenton L. Morgan2
1University of Istanbul, Veterinary Faculty, Department of Microbiology. Avcılar, Istanbul, Turkey
2University of Bristol, School of Veterinary Science, Department of Veterinary Medicine, Langfard, Bristol, U.K
Keywords: IgG, ovine, isolation, antiserum
Abstract
Ovine immunoglobilin G was isolated from sheep serum using gel filtration, Jon-exchange and Gamma Bind-G affinity chromatography. The isolate was electrophoresed using SDS-Page gel electrophorersis.The proteinss in the region between 52-56kD were elecroeluted and rabbit was immunized. Obtained antiserum was analysed using Western Immunoblotting and reacted with a protein in sheep serum in the region 54kD which is identical to IgG heavy chain.
  • Top
  • Abstract
  • [ Başa Dön ] [ Özet ] [ PDF ] [ Benzer Makaleler ] [ Editöre E-Posta ]
    Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim