The aim of this study was to compare the diagnostic value of the laboratory tests used to diagnose urinary tract infection. Urine samples were collected from patients who visited different clinics of a university hospital. A drop of uncentrifuged urine was stained with Gram's stain and examined in microscope. The urine samples were inoculated into appropriate media and considered positive if the growth was more than 104 cfu/ml. The amount of glucose, dansity, pH, erytrocyte, protein, nitrite, ascorbic acid, leucocyte in urine samples were determined by urine dipstik tests. Centrifuged urines were examined in microscope. The sensitivity, specificity, positive and negative predictive values were calculated using urine culture as criterion standard. Females comprised 59.7% of the 575 patients who were enrolled in this study and 33.9% of the patients had urine culture results indicating significant bacteriuria. The most frequently isolated bacteria were E. coli, Klebsiella spp., Staphylococcus spp., Enterococcus spp., Streptococcus spp., respectively. Positive protein, nitrite, erythrocyte, and leukocyte results on dipstik test, and presence of leukocyte and bacteria on Gram staining was found significantly related with urine culture results. Sensitivity and specifity for bacteria and leukocyte on Gram staining were 89,8%, 86,4% and 88,9%, 87,2%, respectively. Negative predictive values of leukocyte on direct microscopic evaluation, bacteria and leukocyte on Gram staining, and erythrocyte, protein, nitrite and leukocyte on dipstick test were found high.