Objective: In this retrospective study it was aimed to evaluate the distribution and antibiotic susceptibility of the microorganisms isolated from wound samples in Kahramanmaras Sutcu Imam University Medical Faculty Hospital, Turkey between January 2008 - January 2011.

Materials and Methods: Wound samples were evaluated by routine culture methods and identification and antibiotic susceptibility testing were done by VITEK 2 automated system (BioMerieux, France).

Results: A total of 525 bacteria, 421 (80.1 %) being gram negative bacteria and 104 (19.8 %) being gram positive, were isolated from the wound samples. The most commonly isolated microorganisms were Esherichia coli (28.5 %), Enterobacter aerogenes (15.6 %), Staphylococcus aureus (14.8 %) and Pseudomonas aeruginosa (14 %). Imipenem was found to be the most susceptible antibiotic for gram negative bacteria. Methicillin resistance was detected in 18.3 % of S. aureus strains and 54.5 % of coagulase negative staphylococci. Vancomycin resistance was not detected among staphylococci. The rate of wound infections was highest in the General Surgery ward.

Conclusion: The distribution and antibiotic suspectibility of the microorganisms in wound infections should be determined periodically in each institution to guide empirical antibiotic treatment.