2013, Cilt 43, Sayı 1, Sayfa(lar) 017-021 |
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Investigation of Secreted Aspartyl Proteinase, Phospholipase, Esterase, and Biofilm Formation in Candida glabrata Strains Isolated from Various Clinical Specimens |
Berna GÜLTEKİN1, Yasin TİRYAKİ2, Neriman AYDIN1 |
1Adnan Menderes Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Aydın 2Aydın Devlet Hastanesi, Mikrobiyoloji Bölümü, Aydın |
Keywords: Candida glabrata, virulence |
Objective: Recent reports suggest that the frequency of Candida glabrata is increasing among species isolated from candidiasis. The aim of this study was to investigate the activities of secreted aspartyl proteinase (SAP), phospholipase, esterase and biofilm formation which might be related to the virulence of C. glabrata strains.
Materials and Methods: The study included the revived C. glabrata storage strains isolated from various clinical specimens in our laboratory. Identification of strains was confirmed by morphological tests and polymerase chain reaction. The SAP activity of the strains was tested in bovine serum albumin agar; the phospholipase activity in egg yolk agar; the esterase activity in Tween-80 agar and the biofilm production was assessed by the microplate method.
Results: A total of 83 C. glabrata strains isolated from vaginal specimens (n=65), blood (n=10), urine (n=4), pus (n=2), and oral swab samples (n=2), were included in the study. Two of the 83 strains (2.4%) were positive for biofilm production (strains from blood and vaginal specimen), while none of the isolates showed SAP, phospholipase or esterase activities.
Conclusion: According to these results, it can be concluded that the virulence factors investigated in this study do not seem to count as very important virulence factors for C. glabrata infections. However, further larger scale studies are needed to explore the virulence factors of C. glabrata.
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