Staphylococcus aureus which is an important pathogen, is known to have several virulence factors including biofilm formation. Biofilm formation is usually associated with chronic infections and has become a subject of interest in a wide area of research. Biofilm is an adherent structure formed by bacteria encased within a matrix produced on natural body surfaces or medical devices. Since biofilm producing S. aureus are more resistant to antibiotics and biofilms prevent phagocytosis, the treatment of biofilm positive S. aureus infections is difficult. The icaADBC locus and some proteins have been shown to be responsible for the formation of S. aureus type biofilm strata. The objective of this study was to summarize the relationships among icaA, icaD and bap genes and between phenotype expressions which are responsible for the formation of biofilm in S. aureus.