Objective: In this study the antibiotic susceptibilities of Acinetobacter baumannii strains isolated in the Microbiology Laboratory of Kahramanmaras Necip Fazıl City Hospital, Kahramanmaras, Turkey, between March 2012 and May 2013, were investigated.

Materials and Methods: Isolation and identification of the A. baumannii isolates were performed by conventional methods and VITEK 2® (bioMérieux, France) automated system.

Results: A. baumannii strains were isolated from the following clinical samples in respective percentages: sputum (40.4%), 36.2% wound scrapings (36.2%), urine (17%) and blood samples (6.4%). These strains were mainly isolated from the intensive care units (49%) and surgery wards (13%). A. baumannii strains tested were susceptible to colistin (100%), netilmicin (21.3%), imipenem (19.2%), tigecycline (14.9 %), cefepime and meropenem (12.7%), to piperacillin-tazobactam and levofloxacin (10.6%), gentamicin ciprofloxacin and cefoperazone-sulbactam (8.5%),, to ampicillin-sulbactam and ceftriaxone (6.4%) in respective percentages.

Conclusion: It was concluded that colistin was the most effective antibiotic for Acinetobacter spp. isolated in our center. High rates of resistance to beta-lactam antibiotics including carbapenems, and other antibiotics were remarkable.