Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2015, Cilt 45, Sayı 4, Sayfa(lar) 181-187
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Investigation of Biofilm Formation of ESBL Positive Uropathogenic Escherichia coli Isolates on (Microplate) Surfaces Modified with Plasma Polymerization Technique and Nanomaterials: An Experimental Model
Elvan HORTAÇ1, Gizem KALELİ2, Dilek ÇÖKELİLER3, Şükran YAVUZDEMİR4, Mehmet MUTLU2, Müge DEMİRBİLEK EKİCİ1, Jülide Sedef GÖÇMEN1
1Başkent Üniversitesi Tıp Fakültesi Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara
2TOBB ETÜ Mühendislik Fakültesi Biyomedikal Mühendislik Bölümü, Ankara
3Başkent Üniversitesi Mühendislik Fakültesi Biyomedikal Mühendislik Bölümü, Ankara
4Atatürk Hastanesi Mikrobiyoloji Laboratuvarı, Ankara
Keywords: Biofilm, nanomaterial, uropathogenic E. coli

Objective: Extended spectrum beta lactamase (ESBL) positive urinary pathogen Escherichia coli is one of the major agents that lead to catheter-associated urinary tract infection. Prevention of colonization via coating the catheter with biocompatible materials produced by biotechnology is an important step in the prevention of infection. In the presented study, it was aimed to investigate the biofilm formation of ESBL positive urinary pathogen E. coli isolates on microplates which were coated with different bio-compatible nano-materials.

Materials and Methods: Biofilm formation of a total of 45 ESBL positive E. coli isolates identified at urine cultures of patients who attended to the medical and surgical outpatient clinics of Ankara TOBB ETU Hospital, on microplates coated with two different monomers [2- hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDM)] using plasma polymerization technique was examined.

Results: Out of 45 ESBL positive E. coli strains used in the study, 8 were observed to form a slight level of biofilm on uncoated plates whereas on EGDM coated microplates 11, and on HEMA coated microplate only 1 strain formed biofilm.

Conclusion: In vitro results showed that using urinary catheters coated with monomer material can reduce catheter associated urinary system infections and morbidity and mortality rates accordingly.


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