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2016, Cilt 46, Sayı 2, Sayfa(lar) 069-075
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Using Galleria mellonella Larvae as the in Vivo Model in Investigating the Secretory Acid Proteinase Activity of Candida albicans
Ali ALVANDİAN1, Mohamad Hasan JAWADİ2, Zeynep Nur ALTINTAŞ2, Naci YILDIZ2, Meral KARAMAN1
1Dokuz Eylül Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İzmir
2Dokuz Eylül Üniversitesi Tıp Fakültesi, Dönem 2 Öğrenci Çalışma Modülü Grubu, İzmir
Keywords: Candida albicans, Galleria mellonella, secretory acid proteinase

Aim: Mouse-rat models have been used for many years in studying the pathogenesis of infectious diseases. Using Galleria mellonella larvae was accepted in the literature as a model host for many microorganisms that are important for medical reseach. In this study, the secretory acid proteinase (SAP) enzyme activity of Candida albicans was investigated on the G. mellonella larvae as a model host.

Material and Method: Two clinical C. albicans strains were used for the infection model, which had positive and negative SAP enzyme activity determined by bovine serum albumin agar method. Healthy G. mellonella larvae of 2-3 cm length and 250- 300 mg weight in the last larval stage, were divided into four groups; control, sham (phosphate buffer), SAP positive and SAP negative (105 CFU/ml) C. albicans-infected group. Larvae were left at 37ºC. Their health conditions were scored after 96 hours and they were sacrificed. Fungal burden was studied.

Results: A significant difference was found between the groups when evaluated in terms of activity, survival, melanization and fungal load of larvae. The control and sham groups showed no death and melanization, no pathogen growth was detected at the end of 96 hours. Fungal burden, melanization and total health score showed significant difference between the SAP positive and SAP negative groups (p<0.05).

Conclusion: In our study, the role of the SAP enzyme activity of C. albicans on the virulence was demonstrated in G. mellonella larvae in vivo model. G. mellonella larvae model can be used as a reliable, easily applicable and an inexpensive method in investigating the effects of fungal infections and virulence factors such as SAP on host cells. Widespread use of the larvae model as an alternative to mammalian models will also contribute to reducing ethical concerns.

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