2016, Cilt 46, Sayı 2, Sayfa(lar) 069-075 |
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Using Galleria mellonella Larvae as the in Vivo Model in Investigating the Secretory Acid Proteinase Activity of Candida albicans |
Ali ALVANDİAN1, Mohamad Hasan JAWADİ2, Zeynep Nur ALTINTAŞ2, Naci YILDIZ2, Meral KARAMAN1 |
1Dokuz Eylül Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İzmir 2Dokuz Eylül Üniversitesi Tıp Fakültesi, Dönem 2 Öğrenci Çalışma Modülü Grubu, İzmir |
Keywords: Candida albicans, Galleria mellonella, secretory acid proteinase |
Aim: Mouse-rat models have been used for many years in
studying the pathogenesis of infectious diseases. Using
Galleria mellonella larvae was accepted in the literature as a
model host for many microorganisms that are important for
medical reseach. In this study, the secretory acid proteinase
(SAP) enzyme activity of Candida albicans was investigated on
the G. mellonella larvae as a model host.
Material and Method: Two clinical C. albicans strains were
used for the infection model, which had positive and negative
SAP enzyme activity determined by bovine serum albumin agar
method. Healthy G. mellonella larvae of 2-3 cm length and 250-
300 mg weight in the last larval stage, were divided into four
groups; control, sham (phosphate buffer), SAP positive and SAP
negative (105 CFU/ml) C. albicans-infected group. Larvae were
left at 37ºC. Their health conditions were scored after 96 hours
and they were sacrificed. Fungal burden was studied.
Results: A significant difference was found between the groups
when evaluated in terms of activity, survival, melanization and
fungal load of larvae. The control and sham groups showed no
death and melanization, no pathogen growth was detected at
the end of 96 hours. Fungal burden, melanization and total
health score showed significant difference between the SAP
positive and SAP negative groups (p<0.05).
Conclusion: In our study, the role of the SAP enzyme activity
of C. albicans on the virulence was demonstrated in
G. mellonella larvae in vivo model. G. mellonella larvae model
can be used as a reliable, easily applicable and an inexpensive
method in investigating the effects of fungal infections and
virulence factors such as SAP on host cells. Widespread use of
the larvae model as an alternative to mammalian models will
also contribute to reducing ethical concerns.
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