2017, Cilt 47, Sayı 2, Sayfa(lar) 078-082 |
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|
Use of Carbapenemase Inactivation Test in the Detection of Carbapenemase Production Among The Isolates of Carbapenem- Resistant Klebsiella pneumoniae |
Tayfun DEMİRAY1, Özlem AYDEMİR1, Ümit KILIÇ2, Kerem YILMAZ2, Mehmet KÖROĞLU2, Mustafa ALTINDİŞ2 |
1Sakarya Üniversitesi, Eğitim ve Araştırma Hastanesi, Klinik Mikrobiyoloji Laboratuvarı, Sakarya 2Sakarya Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Sakarya |
Keywords: Carbapenemase inactivation test, carbapenemases, Klebsiella pneumoniae |
Objective: The infections caused by carbapenemaseproducing
Enterobacteriaceae isolates are among the most
actual health problems in our country as well as all over
the world. Epidemics and sporadic cases caused by
carbapenem- resistant Klebsiella species have begun to be
reported increasingly from our country. In this study, we
aimed to compare the diagnostic value of carbapenemase
inactivation test with the modified Hodge test in
carbapenemase producing K. pneumoniae isolates verified
by molecular method and to evaluate the use of this test in
routine laboratory practice.
Material and Methods: A total of 125 isolates of Klebsiella
pneumoniae harvested from clinical, and screening
specimens between 2014 and 2015 were evaluated.
Carbapenemase-producing 60, and carbapenem sensitive
60 isolates were used for the evaluation of the modified
Hodge test and carbapenemase inactivation test. Molecular
evaluation was performed by investigating the blaIMP-1,
blaKPC, blaNDM-1, blaOXA-48 and blaVIM gene regions with
Carba R kits (Cepheid, USA) of the Gene-Xpert® system.
Results: The modified Hodge test detected 54 out of 60
Klebsiella isolates, which were found to have carbapenemase
encoding gene by molecular method; whereas carbapenemase
inactivation test detected 52 out of 60 isolates.
Conclusion: When molecular method is regarded as gold
standard, sensitivities of modified Hodge test and
carbapenemase inactivation test were 0.85 and 0.80;
specificities were calculated as 0.95 and 0.93, respectively.
Phenotypic tests depending on enzyme inhibition can be
preferred in routine practice since they have low cost and
are easy-to-apply. However, it should not be forgotten that
the sensitivity and specificity of these tests are variable.
|
[ Türkçe Özet ]
[ PDF ]
[ Benzer Makaleler ]
|