Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2018, Cilt 48, Sayı 1, Sayfa(lar) 038-044
[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Investigation of In Vitro Susceptibility of Non-albicans Candida Species Isolated from Clinical Specimens to Fluconazole, Itraconazole, Voriconazole by Reference Broth Microdilution Method: Application of New Species-Specific Clinical Breakpoints and Epidemiological Cutoff Values
Gülşen HAZIROLAN
Ankara Numune Eğitim ve Araştırma Hastanesi, Ankara
Keywords: Non-albicans Candida, fluconazole, itraconazole, voriconazole, antifungal susceptibility profile

Objective: The aim of this study was to determine in vitro antifungal susceptibility profiles of the most frequently isolated non-albicans Candida species to fluconazole, itraconazole and voriconazole in our hospital.

Material and Methods: Non-albicans Candida species isolated from the clinical specimens of patients who were hospitalized in Ankara Training and Research Hospital between January 2013 and June 2016 were included in the study. Isolates were identified by conventional methods and MALDI-TOF MS. In vitro antifungal susceptibilities of isolates were determined by Clinical and Laboratory Standards Institute (CLSI, M27-A3) reference broth microdilution method (BMD). Minimal inhibitor concentration values were evaluated with new species- specific clinical breakpoints and epidemiological cut-off values.

Results: The species distribution of the isolates were as follows; C. glabrata (n=37), C. parapsilosis (n=24), C. krusei (n=24), C. tropicalis (n=14), C. lusitaniae (n=13), C. kefyr (n= 10), C. guilliermondii (n=2) and C. dubliniensis (n=2). It was determined that two of C. glabrata isolates were resistant to fluconazole and one C. parapsilosis isolate was dose dependent susceptible to fluconazole, one C. parapsilosis and one C. tropicalis isolates were non-wild type to itraconazole, one C. krusei and two C. parapsilosis isolates were voriconazole intermediate, and two C. glabrata, five C. kefyr and three C. lusitaniae isolates were non-wild type to voriconazole.

Conclusion: In our study, 126 non- albicans Candida isolates were tested for in vitro antifungal susceptibility profiles, two isolates were detected as resistant to triazole and 12 isolates were evaluated as non-wild type to triazoles. No cross- resistance was observed for tiazoles in these isolates. These data emphasize the necessity of performing antifungal susceptibility tests for nonalbicans Candida isolates at each center in order to direct the treatment properly.


[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim