2018, Cilt 48, Sayı 1, Sayfa(lar) 078-085 |
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Comparison of Results of Urine Cultures and a Flow Cytometric Device That Analyses Urinary Leukocytes/ Bacteria |
Emel ÜZMEZ1, Serap YAĞCI1, Mihriban YÜCEL1, Buğra Bilge KESEROĞLU2, Ayşe Esra KARAKOÇ1, Bedia DİNÇ1 |
1Ankara Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Ankara 2Ankara Eğitim ve Araştırma Hastanesi, Üroloji Kliniği, Ankara |
Keywords: Comparison: flow cytometry and urine cultures |
Objective: It was aimed to make a fast screening algorithm
by comparing the results obtained by the flow cytometry
method with the urine culture results so as to to eliminate
cultivation in samples containing a number of leukocytes
and bacteria below a threshold value. In the second step of
the study; it was evaluated to what extent unnecessary
antibiotic usage could be avoided by finalizing the results
of negative-contaminated urine specimens in a short-term.
Material and Methods: The urine samples of 995 patients
from all clinics submitted to our laboratory for culture
were also tested in a flow cytometry device. The results
obtained from the flow cytometry device were compared
with the culture results. In the second step, the use of
antibiotics in 208 patients referred from the urology clinic
were recorded, in order to evaluate the empirical use of
antibiotics, which were initiated without the culture results
were obtained.
Results: The results we obtained with the flow cytometry
method showed that there was no need to carry out urine
culture in 31% of our samples that were sent from all the
clinics. As to the samples obtained from the urology clinic, it
was detected that there was no need to carry out urine culture
in 29.3%; and 23% of the patients whose urine cultures were
detected as no growth on flow cytometry predicted had
been started on unnecessary antibiotic therapy.
Conclusion: When this technique is used as a rapid
screening test, it will reduce the workload and cost in
laboratories in which urine specimens are frequently sent for
analysis; also contribute to a certain extent in reducing
unnecessary antibiotic use by providing negative results
within minutes.
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