2018, Cilt 48, Sayı 4, Sayfa(lar) 235-240 |
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Detection of EBV-DNA in Children With Prolonged Lymphadenopathy |
Şule DURMUŞ1, Mustafa ÖNEL1, Emin ÜNÜVAR2, Ali AĞAÇFİDAN2 |
1İstanbul Üniversitesi İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul 2İstanbul Üniversitesi İstanbul Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, İstanbul |
Keywords: Epstein-Barr virus (EBV), lymphadenopathy, real-time PCR |
Objective: Epstein-Barr virus (EBV) is a common virus all
around the world with observed seropositivity of 90% in
adult population. More than 90% of the children are
infected with this virus before the age of 6. This study aims
to determine the quantitative viral load of EBV DNA in
children between 0-18 years of age with prolonged
lymphadenopathy.
Material and Methods: Among the patients with
lymphadenopathy symptoms, who were admitted to the
Istanbul University, Department of General Pediatrics
between the years 2014 and 2015, 100 volunteer children
between the age of 0-18 whose informed consents were
taken from their parents were included in the study. Viral
DNA was isolated from the whole blood samples of the
patients for Real Time PCR (RT-PCR) method.
Results: EBV DNA was found in 33 (33%) of the patients.
Of the patients who were positive for EBV DNA 17 (51.5%)
were male while the remaining 16 patients (48.5%) were
female. The highest quantitative value of EBV DNA was
found in patients between the ages of 0 and 6.
Conclusion: Despite serological tests were preferred as the
first-line tests between 0-4 years of age, the results of our
study suggest that viral load may have an important role in
the diagnosis by quantitative determination and assessment
of EBV DNA. In order to prevent potentially undesirable
clinical manifestations that may arise from EBV which is an
oncogenic virus, in later years, it is necessary to keep in
mind that evaluation of viral load EBV DNA should be
performed in children who have prolonged lymphadenopathy
complaints.
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