Objective: The aim of this study was to evaluate retrospectively results of the IgM and IgG antibody seropositivity and IgG avidity tests performed in serum samples obtained from patients with suspected Toxoplasma gondii, rubella and Cytomegalovirus (CMV) infections or for routine control for pregnancy follow up which were sent to Medical Microbiology Serology/ELISA Laboratory of Cerrahpaşa Faculty of Medicine between January 2013 and December 2018 with various clinical indications.

Method: T. gondii, rubella and CMV IgM antibodies examined by immunocapture ELISA method and T. gondii, rubella and CMV IgG antibodies detected by indirect ELISA method in serum samples of 84,807 patients who applied to Laboratories of Istanbul University Cerrahpaşa Faculty of Medicine Hospital between 2013-2018 were evaluated. In addition, IgG avidity test results were examined in serum samples of T. gondii, rubella and CMV IgG avidity tests were performed using indirect ELISA tests realized in 916 cases.

Results: In the study, IgM / IgG positivities for T. gondii, rubella and CMV were found to be 2.2% / 97.8%, 0.24% / 99.7% and 3.1%/ 94%, respectively. Avidity index tests for all three agents were requested for 274 (30%) cases with both IgM and IgG- positivities The detection rate of low avidity indices for T. gondii, rubella and CMV were 44 (16%), 9 (3.2%) and 8 (2.9%) respectively.

Conclusion: Multiple molecular tests provide a better understanding of the role of viral agents in respiratory infections in adults. We think that the detection of the viral agent with the diagnosis of the agent targeted in these infections will be beneficial in preventing unnecessary use of antibiotics with the correct treatment application and will provide fast and accurate management decisions for infection control measures.