Objective: Tuberculosis is an airborne infectious disease caused by the members of the Mycobacterium tuberculosis complex (MTBC) and is a significant public health problem. Our study aims to determine the species of MTBC isolates obtained from our laboratory and investigate mutations associated with drug resistance.

Methods: We analyzed 55 MTBC isolates obtained from clinical samples. We used the GenoType MTBC assay (Hain-Lifescience, Germany) for species determination and the GenoType MTBDRplus assay (Hain-Lifescience, Germany) to detect mutations associated with isoniazid and rifampicin resistance, following the manufacturer’s recommendations.

Results: Out of the 55 MTBC isolates, 39 (70.9%) were identified as M. tuberculosis / M. canettii, 11 (20%) as M. bovis, and 5 (9.1%) as M. caprae. We detected the katG MUT1 mutation in two isolates and the inhA MUT1 mutation in two isolates. The rpoB MUT3 mutation was found in three isolates.

Conclusion: In our region, the majority of human tuberculosis cases are caused by M. tuberculosis / M. canettii. However, zoonotic species like M. bovis and M. caprae are also significantly present. Our isolates contain rpoB MUT3, katG MUT1 and inhA MUT1 mutations associated with drug resistance.