Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim  
2013, Cilt 43, Sayı 3, Sayfa(lar) 090-096
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Direct Examination and Culture Results of Scraping Specimens Sent to Mycology Laboratory of Dokuz Eylül University Medical Faculty Hospital
M. Cem ERGON, Müjgan ÖZHUN, Mine DOLUCA DERELİ
Dokuz Eylül Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı
Keywords: Dermatophytes, scraping specimens, culture

Objective: It was aimed to analyze retrospectively the results of direct examination and culture of nail, skin and hair/scalp scraping specimens sent to Mycology Laboratory of Dokuz Eylül University Medical Faculty Hospital within the last five years.

Materials and Methods: Direct examination and culture- ordered 2533 specimens and only direct examination-ordered 655 specimens were analyzed between the period of January 2008 and June 2012. Microscopic examination of the specimens was performed using 15% potassium hydroxide and the specimens were cultivated on Sabouraud dextrose agar and Mycosel agar media , and incubated at 30ºC for four weeks. The identification of the isolates was performed by conventional methods.

Results: Fungal elements were observed in 843 (33.28%) of 2533 direct examination and culture- ordered specimens. Of all the specimens, 30 (1.19%) were reported as contamination, while in 278 (10.98%) dermatophyte growth and in 31 (1.22%) growth of non-dermatophyte mold or yeast were observed. In 248 (29.42%) of the specimens in which hyphae/hyphae and spores were observed by microscopic examination, growth of dermatophytes was determined. In 30 (10.80%) of the specimens in which dermatophytes grew, fungal elements were not observed by direct examination. The most common agents were determined as Trichophyton rubrum (74.10%), T. mentagrophytes (9.71%) and Microsporum canis (7.19%). Although a significant increase in the rate of T. rubrum isolation was observed within years, a statistically significant difference as for distribution of species was not detected. In 236 (36.03%) of the only direct examination- ordered specimens, fungal elements were observed. Although most prevalently specimens of nail were sent for direct examination and culture (59.73%), dermatophyte growth was mostly observed among skin specimens(53.96%) in our study. The fungal growth rate in skin specimens was statistically significantly higher than those detected in nail specimens (χ2=34.00; p<0.0001).

Conclusion: The distribution of the most frequently isolated fungal species from the specimens sent to our laboratory and their frequencies were compatible to the data obtained previously in our country and the world.


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Ana Sayfa | Dergi Hakkında | Yayın Kurulu | Telif Hakkı Devir Formu | Arşiv | Yayın Arama | Yazarlara Bilgi | Etik Politikalar | İletişim