2000, Cilt 30, Sayı 1-2, Sayfa(lar) 056-059 |
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Isolation and Preparation of Antisera to Ovine IgG Heavy Chain |
Hüseyin Yılmaz1, Nuri Turan1, Kenton L. Morgan2 |
1University of Istanbul, Veterinary Faculty, Department of Microbiology. Avcılar, Istanbul, Turkey 2University of Bristol, School of Veterinary Science, Department of Veterinary Medicine, Langfard, Bristol, U.K |
Keywords: IgG, ovine, isolation, antiserum |
Ovine immunoglobilin G was isolated from sheep serum using gel filtration, Jon-exchange and Gamma Bind-G affinity chromatography. The isolate was electrophoresed using SDS-Page gel electrophorersis.The proteinss in the region between 52-56kD were elecroeluted and rabbit was immunized. Obtained antiserum was analysed using Western Immunoblotting and reacted with a protein in sheep serum in the region 54kD which is identical to IgG heavy chain.
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